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Histidine is an amino acid. Amino acids are the building blocks of protein in our bodies. People use histidine as medication.

Some people take histidine by mouth for metabolic syndrome, diarrhea brought on by cholera infection, rheumatoid arthritis, allergic diseases, ulcers, and anemia triggered by kidney failure or kidney dialysis. [2]


Histidine hĭs ´ tĭdēn [crucial], organic compound, among the 22 α-amino acids frequently found in animal proteins. Only the l-stereoisomer appears in mammalian protein. Histidine is the direct precursor of histamine; it is likewise a crucial source of carbon atoms in the synthesis of purines. The imidazole group on the side chain of histidine can serve as both an acid and a base, i.e., it can both donate and accept protons under some conditions. This turns out to be an important property when histidine is incorporated into proteins, especially when it becomes a part of the primary structure of some enzymes. It is thought that the side chain of this amino acid functions as a general acid and base as it takes part in the catalytic functions of chymotrypsin, in addition to those of a variety of enzymes dealing with the metabolic process of carbs, proteins, and nucleic acids. It has actually even been linked in the operations of cocoonase, the enzyme that permits adult silk moths to escape from their cocoons. Histidine is thought about to be a vital amino acid for infants (it should be supplied in the diet plan); experiments with adults indicate that they can opt for at least brief periods without dietary consumption of this amino acid. It was separated from protein in 1896; its structure was confirmed by chemical synthesis in 1911. [3]

Metabolic process


Histidine biosynthesis pathway 8 various enzymes can catalyze ten responses. In this image, his4 catalyzes 4 different reactions in the path.

L-histidine is an important amino acid that is not synthesized de novo in people. People and other animals should consume histidine or histidine-containing proteins. The biosynthesis of histidine has been widely studied in prokaryotes such as e. Coli. Histidine synthesis in e. Coli involves eight gene items (his1, 2, 3, 4, 5, 6, 7, and 8) and it happens in 10 steps. This is possible because a single gene product has the ability to catalyze more than one reaction. For example, as displayed in the path, his4 catalyzes 4 different steps in the path.

Histidine is synthesized from phosphoribosyl pyrophosphate (prpp), which is made from ribose-5-phosphate by ribose-phosphate diphosphokinase in the pentose phosphate pathway. The very first reaction of histidine biosynthesis is the condensation of prpp and adenosine triphosphate (atp) by the enzyme atp-phosphoribosyl transferase. Atp-phosphoribosyl transferase is shown by his1 in the image. His4 gene item then hydrolyzes the item of the condensation, phosphoribosyl-atp, producing phosphoribosyl-amp (pramp), which is an irreparable step. His4 then catalyzes the development of phosphoribosylformiminoaicar-phosphate, which is then converted to phosphoribulosylformimino-aicar-p by the his6 gene product. His7 divides phosphoribulosylformimino-aicar-p to form d-erythro-imidazole-glycerol-phosphate. After, his3 types imidazole acetol-phosphate releasing water. His5 then makes l-histidinol-phosphate, which is then hydrolyzed by his2 making histidinol. His4 catalyzes the oxidation of l-histidinol to form l-histidinal, an amino aldehyde. In the last action, l-histidinal is transformed to l-histidine.

Just like animals and bacteria, plants need histidine for their growth and development. Bacteria and plants are similar because they can synthesize histidine. Both synthesize histidine from the biochemical intermediate phosphoribosyl pyrophosphate. In general, the histidine biosynthesis is extremely comparable in plants and microbes.

Policy of biosynthesis

This pathway needs energy in order to take place for that reason, the existence of atp triggers the first enzyme of the path, atp-phosphoribosyl transferase. Atp-phosphoribosyl transferase is the rate identifying enzyme, which is managed through feedback inhibition meaning that it is inhibited in the existence of the item, histidine.


Histidine is among the amino acids that can be converted to intermediates of the tricarboxylic acid (tca) cycle (also called the citric acid cycle). Histidine, along with other amino acids such as proline and arginine, participates in deamination, a process in which its amino group is gotten rid of. In prokaryotes, histidine is first converted to urocanate by histidase. Then, urocanase transforms urocanate to 4-imidazolone-5-propionate. Imidazolonepropionase catalyzes the reaction to form formiminoglutamate (figlu) from 4-imidazolone-5-propionate. The formimino group is moved to tetrahydrofolate, and the staying five carbons form glutamate. Overall, these responses result in the development of glutamate and ammonia. Glutamate can then be deaminated by glutamate dehydrogenase or transaminated to form α-ketoglutarate. [4]


Chemical homes:

Standard (fundamental group).

Physical properties:

Polar (positively charged).

Histidine, an important amino acid, has as a positively charged imidazole functional group.

The imidazole makes it a typical individual in enzyme catalyzed reactions. The un protonated imidazole is nucleophilic and can serve as a basic base, while the protonated form can serve as a basic acid. The residue can likewise serve a function in stabilizing the folded structures of proteins. [5]

System of action

Given that the actions of supplemental l-histidine are uncertain, any postulated mechanism is entirely speculative. However, some facts are known about l-histidine and a few of its metabolites, such as histamine and trans-urocanic acid, which suggest that additional l-histidine may one day be revealed to have immunomodulatory and/or antioxidant activities. Low totally free histidine has actually been found in the serum of some rheumatoid arthritis patients. Serum concentrations of other amino acids have actually been found to be regular in these patients. L-histidine is an excellent chelating agent for such metals as copper, iron and zinc. Copper and iron take part in a reaction (fenton reaction) that creates powerful reactive oxygen types that could be damaging to tissues, including joints. L-histidine is the obligate precursor of histamine, which is produced through the decarboxylation of the amino acid. In experimental animals, tissue histamine levels increase as the amount of dietary l-histidine boosts. It is likely that this would hold true in humans too. Histamine is known to have immunomodulatory and antioxidant activity. Suppressor t cells have h2 receptors, and histamine triggers them. Promotion of suppressor t cell activity could be advantageous in rheumatoid arthritis. Further, histamine has actually been shown to down-regulate the production of reactive oxygen species in phagocytic cells, such as monocytes, by binding to the h2 receptors on these cells. Decreased reactive oxygen types production by phagocytes might play antioxidant, anti-inflammatory and immunomodulatory roles in such diseases as rheumatoid arthritis. This latter mechanism is the rationale for using histamine itself in a number of medical trials studying histamine for the treatment of particular types of cancer and viral diseases. In these trials, down-regulation by histamine of reactive oxygen types development appears to prevent the suppression of natural killer (nk) cells and cytotoxic t lymphocytes, permitting these cells to be more reliable in assaulting cancer cells and virally contaminated cells. [6]


Amino acids are categorized into three groups:.

  1. Important amino acids
  2. Unnecessary amino acids
  3. Conditional amino acids

Essential amino acids

Essential amino acids can not be made by the body. As a result, they must originate from food.

The 9 important amino acids are: histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, and valine.

Excessive amino acids

Inessential methods that our bodies can produce the amino acid, even if we do not get it from the food we eat. Excessive amino acids consist of: alanine, arginine, asparagine, aspartic acid, cysteine, glutamic acid, glutamine, glycine, proline, serine, and tyrosine.

Conditional amino acids

Conditional amino acids are typically not essential, other than in times of disease and stress.

Conditional amino acids consist of: arginine, cysteine, glutamine, tyrosine, glycine, ornithine, proline, and serine.

You do not need to eat necessary and excessive amino acids at every meal, however getting a balance of them over the whole day is important. A diet plan based on a single plant item will not be adequate, however we no longer fret about pairing proteins (such as beans with rice) at a single meal. Instead we take a look at the adequacy of the diet plan overall throughout the day. [7]

Function of histidine

Histidine is utilized by the body to make particular hormonal agents and metabolites that affect kidney function, transmission of nerves, stomach secretions, and the immune system. Histidine likewise has an effect on the repair work and growth of tissue, making blood cells and helping to protect afferent neuron. It is also utilized to make histamine in the body.

A main function of histidine in the body is to manage and assist metabolize (break down and utilize for energy) trace elements. These micronutrient consist of:.

Histidine likewise helps to form many different enzymes and compounds in the body. In addition, histidine works to develop a substance called metallothionein inside of the cells of the brain, liver, and kidneys; metallothionein secures the brain cells and needs histidine to be formed. If a person’s body is hazardous with heavy metals (such as mercury and lead), it may lead to a depletion of sufficient shops of histidine.

Allergic reactions and histidine

The body utilizes histidine to make histamine (a common cause of swelling and itching that takes place as a result of an allergy) as a response to allergies or tissue damage.

Histamine– discovered in raised levels during an allergic reaction– is a by-product of histidine. Histamine causes the immune system to introduce an inflammatory reaction (consisting of itching and swelling) as a reaction to allergens.

Histidine contributes to an emergency situation (and potentially fatal) medical condition called anaphylaxis that can arise from an allergic reaction. It is treated with an injection of epinephrine. [8]

Health advantages

Effective for:

Surgical procedures

Bretschneider’s histidine-tryptophan-ketoglutarate option (htk) is a histidine-containing buffering solution regularly used to induce heart arrest during surgical procedures and safeguard the heart muscle from low blood supply.

Numerous medical trials vouch for its effectiveness to decrease damage due to low oxygen in not only the heart, however also the kidneys.

The service is also utilized to protect donor organs.

Inadequate proof for:

The following purported advantages are just supported by limited, low-grade clinical research studies and some animal and cell-based research. There is insufficient proof to support using histidine supplements for any of the below-listed usages until larger, more robust clinical trials are performed. Keep in mind to speak with a medical professional before taking histidine supplements. They ought to never ever be utilized as a replacement for approved medical treatments.

Safeguarding the heart

Mutations resulting in increased histidine levels were related to lower occurrence of coronary heart disease in an observational study on over 1,100 african americans.

The histidine derivative carnosine enhanced workout performance and lifestyle in a clinical trial on 50 individuals with congestive heart failure.

Damaged rat hearts (due to brought back blood supply after a heart stroke) treated with histidine revealed better healing. Histidine presumably minimized reactive oxidative species and assisted protect energy (atp).

In diabetic mice, supplementation with carnosine decreased blood fat levels and plaque accumulation in the arteries.

Minimizing high blood pressure

Dietary histidine was associated with lower blood pressure, particularly at higher dosages, in a research study on 92 individuals with heart disease.

In a study in rats with elevated blood pressure, oral histidine supplements considerably lowered it. Likewise, carnosine decreased blood pressure in overweight rats.


In a research study including 92 overweight females with histidine deficiency, supplementing this amino acid over 12 weeks minimized oxidative tension.

Another research study on over 400 women discovered an association between low histidine levels and oxidative tension. In addition, overweight females had even worse antioxidant status, perhaps due to their abnormal histidine and arginine metabolism.


In 2 research studies on over 500 women, histidine supplementation resulted in reduced inflammation by obstructing the production of inflammatory cytokines.

Blood sugar levels

In a scientific trial on 92 obese women with metabolic syndrome, histidine supplements (4 g/day for 12 weeks) considerably reduced insulin resistance.

An observational study on 88 obese individuals associated greater dietary histidine with lower fasting blood sugar levels and increased insulin sensitivity.

In mice, supplements with histidine and carnosine helped prevent diabetic problems.

Brain function

In a clinical trial on 20 individuals with chronic fatigue and sleep disruptions, supplements with histidine for 2 weeks enhanced attention, memory, and clarity of thinking while decreasing tiredness.

In another trial on 25 gulf war i veterans, carnosine treatment improved cognitive function.

In rats, histidine supplementation improved short-term memory and safeguarded the brain from the damage caused by decreased oxygen supply (cerebral ischemia).

Weight problems

An observational research study on 88 obese people associated greater dietary histidine with a decreased body mass index (bmi), waist circumference, and blood pressure.

Histidine is transformed to histamine in the brain. In rats, histidine supplementation decreased their feeding behavior through its conversion to histamine. In another research study, histidine supplementation minimized not just feeding, however also fat accumulation.

However, histidine was inefficient as a cravings suppressant in an old clinical trial.

Skin protection

Histidine is a precursor of urocanic acid, a compound that develops in human skin cells and takes in uv radiation. By doing so, urocanic acid serves as a “natural sunscreen” that may protect dna from sunshine.

In a scientific trial on 24 individuals with eczema, supplementation with histidine for 4 weeks considerably reduced disease severity and 39% of patients reported feeling “better”.

2 research studies in mice discovered increased urocanic acid levels on the skin after histidine supplements, leading to increased protection from uv-radiation.

Preventing embolism

In a medical trial on 18 individuals with increased formation of blood clots (spontaneous platelet aggregation), supplements with histidine for a week avoided blood clots. The impacts were probably moderated by the action of arachidonic acid metabolites.

Potentially ineffective for:


Eye drops with n-acetylcarnosine, a dipeptide composed of histidine and beta-alanine, are often advertised to enhance cataracts without the need for surgery. Nevertheless, a meta-analysis stopped working to find sufficient evidence to back this claim.

Histidine supplements avoided the advancement of cataracts in salmons.

Animal and cell research (lack of proof)

No clinical evidence supports the use of histidine supplements for any of the conditions noted in this section. Below is a summary of the existing animal and cell-based research, which need to assist more investigational efforts. However, the studies need to not be interpreted as encouraging of any health advantage.

Wilson’s disease

Wilson’s illness is a rare genetic disease that triggers excessive copper buildup in the organs, particularly the liver. In a research study in rats, a diet including excess histidine flushed the excess of liver copper out with urine.


In rats, histidine injections reduced the severity of seizures. The authors believed that the impact was due to the function of histidine as a precursor to histamine, a seizure inhibitor.

Limitations and caveats

Very few scientific trials, many of them on little populations, have been carried out. Larger, more robust scientific trials are required to validate the potential health advantages of histidine supplementation for a lot of conditions.

Furthermore, a number of research studies integrated histidine with other compounds, making the specific contribution of histidine to the effects observed challenging to estimate. [9]

Histidine shortage

Indications & signs

Histidinemia is thought about a benign condition. For years, intellectual impairment and speech conditions were related to histidinemia. Nevertheless, these findings are now thought about coincidental and not due to the metabolic flaw of histidinemia because reports of follow-up from newborn screening have shown that the majority of infants with histidinemia do not develop scientific signs (asymptomatic). Nevertheless, clinical symptoms have been reported in some clients with histidinemia. To reconcile this with the benign findings from newborn screening, it has been suggested that histidinemia might be a threat factor for the development of cns issues, and that such problems may establish only in an undesirable situation such as an irregular perinatal occasion.

Individuals with histidinemia have elevated levels of the amino acid histidine in the blood and extreme quantities of histidine, imidazole pyruvic acid, and other imidazole metabolic process items in the urine. The majority of people with histidinemia adjust to the existence of extreme histidine in the blood and do not suffer any ill impacts.

According to the medical literature, babies born to mothers with histidinemia (maternal histinemia) have actually displayed no symptoms.


Histidinemia is acquired in an autosomal recessive pattern. Genetic diseases are determined by 2 genes, one received from the father and one from the mother.

Recessive congenital diseases take place when a specific inherits an irregular variant of a gene from each parent. If a specific receives one regular gene and one abnormal alternative gene for the illness, the individual will be a carrier for the disease, but typically will not show symptoms. The threat for two provider parents to both pass the abnormal version gene and, therefore, have an affected child is 25% with each pregnancy. The threat to have a kid who is a carrier, like the parents, is 50% with each pregnancy. The chance for a kid to get normal genes from both moms and dads is 25%. The risk is the same for males and females. [10]

Side effects

While it’s not likely that you ‘d consume very high quantities of histidine from foods alone, it’s possible to consume excess quantities from supplements, which can cause specific side effects. Studies have found that when individuals take extremely high dosages of histidine, around 32 grams/day or more, they can experience adverse effects like muscle weakness, sleepiness and tiredness, headaches, digestive concerns like nausea and anorexia nervosa, anxiety, and bad memory. A few of these may be because of negative nitrogen balance.

Other unfavorable impacts tied to high histidine levels have actually likewise been shown in animal studies, however it’s unknown how these results rollover to people. In studies involving rats, complications connected to high histidine levels in the brain and liver have actually consisted of copper deficiency, decreased liver function, high cholesterol and loss of appetite.

A few of the possible adverse effects of consuming too much protein in general include weight gain, kidney concerns, irregularity and bad breath. Anyone with kidney or liver illness need to not consume big quantities of amino acids without working with a doctor. [11]

Food sources and suggested consumption

Due to the fact that your body can not produce vital amino acids, it is essential to get them through your diet.

Lots of foods are abundant in vital amino acids, making it simple to satisfy your day-to-day requirements.

Here are the everyday needed intakes for the important amino acids, according to the world health organization. These are for grownups per 2.2 pounds (1 kg) of body weight:.

To find out how much you ought to take in per day, you can multiply the numbers given above by your weight in kgs. For example, an individual who weighs 60 kg (132 pounds) need to take in 1,200 mg (1.2 grams) of isoleucine daily.

Satisfying these requirements is very easy with the majority of diet plans, so there’s normally no requirement to track your consumption of private amino acids.

For example, one 174-gram piece of braised chicken breast supplies 55.9 grams of total protein, easily meeting or surpassing the needs listed above.

Food sources

Foods which contain all 9 necessary amino acids are described as total proteins.

The following foods are total protein sources:.

  • Meat
  • Seafood
  • Poultry
  • Eggs
  • Dairy products

Soy and pea protein are plant-based total protein sources.

Other plant-based sources of protein, such as beans, nuts, and particular grains, are thought about insufficient proteins because they lack one or more of the vital amino acids.

Nevertheless, if you’re following a plant-based diet, you can still guarantee correct consumption of all 9 essential amino acids by eating a range of plant proteins each day.

For example, choosing a variety of plant-based proteins, such as beans, nuts, seeds, whole grains, and vegetables, can make sure that you meet your vital amino acid requires, even if you pick to exclude animal items from your diet.


Numerous animal and plant foods, such as meat, eggs, quinoa, and soy, contain all 9 vital amino acids and are thought about complete proteins. [12]

Special safety measures and cautions

Pregnancy and breast-feeding: insufficient is learnt about the use of histidine during pregnancy and breast-feeding. Remain on the safe side and avoid use.

Folic acid shortage: if you have this condition, don’t utilize histidine. It can trigger an undesirable chemical called formiminoglutamic acid (figlu) to develop in the body. [13]


His has distinct chemical and metabolic residential or commercial properties that are the basis for its use as a treatment for a large range of conditions. His-rich solutions have clear advantages in the preservation of organs for transplant and myocardial defense in cardiac surgical treatment. Further studies are required to illuminate the impacts on muscle tiredness during strenuous workout, neurological conditions, metabolic syndrome, atopic dermatitis, uraemic anaemia resistant to erythropoietin therapy, and inflammatory bowel diseases and as a supplement to increase the effectiveness of methotrexate in treatment of malignancies.

Indications of toxicity, mutagenic activity, and allergies have actually not been reported. Of issue should be reports of hepatic enhancement, increases in ammonia and glutamine levels, and reduces in bcaa levels, showing that his supplementation might be improper in clients with liver disease.

In conclusion, his-containing supplements seem safe and effective substances with an appealing healing capacity in incredibly large number of conditions. Randomized regulated intervention trials in human beings using his-containing substances are called for to confirm their efficiency for particular conditions. [14]


  1. Https://www.merriam-webster.com/dictionary/histidine
  2. Https://www.webmd.com/vitamins/ai/ingredientmono-467/histidine
  3. Https://www.infoplease.com/encyclopedia/science/biochemistry/concepts/histidine
  4. Https://en.wikipedia.org/wiki/histidine
  5. Http://www.biology.arizona.edu/biochemistry/problem_sets/aa/histidine.html
  6. Https://go.drugbank.com/drugs/db00117
  7. Https://medlineplus.gov/ency/article/002222.htm
  8. Https://www.verywellhealth.com/histidine-4777164#toc-what-is-histidine-used-for
  9. Https://supplements.selfdecode.com/blog/histidine/
  10. Https://rarediseases.org/rare-diseases/histidinemia/
  11. Https://draxe.com/nutrition/histidine-benefits/
  12. Https://www.healthline.com/nutrition/essential-amino-acids#food-sources-recommended-intake
  13. Https://www.rxlist.com/histidine/supplements.htm#specialprecautionswarnings
  14. Https://www.ncbi.nlm.nih.gov/pmc/articles/pmc7146355/
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